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Influence of high‐fructose feeding on functional α 1 ‐adrenoceptor subtypes in the rat kidney
Author(s) -
Abdulla Mohammed H.,
Johns Edward J,
Sattar Munavvar A.,
Abdullah Nor A.,
Khan Md. Abdul Hye,
Rathore Hassaan A
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.665.13
Subject(s) - methoxamine , medicine , endocrinology , phenylephrine , vasoconstriction , angiotensin ii , fructose , kidney , adrenergic , renal blood flow , blood pressure , chemistry , agonist , receptor , biochemistry
This study investigated whether the α 1 ‐adrenoceptor responsiveness of the renal vasculature was altered in a metabolic syndrome state due to high‐fructose feeding. Male Sprague‐Dawley rats were fed for 8 weeks with 20% fructose in the drinking water (FFR). They were pentobarbitone anaesthetized and the reductions in renal cortical blood flow to intrarenal administration of noradrenaline, phenylephrine, methoxamine and angiotensin II (Ang II) were determined before and after 5‐methylurapidil, chloroethylclonidine (CEC) or BMY 7378. Data, mean±SEM were subjected to ANOVA with significance at P<0.05. FFR had higher systolic blood pressure, plasma glucose and insulin level as compared to control (FFR vs. control, 123±2 vs. 107±1 mmHg, 6.8±0.3 vs. 5±0.3 mmol/L and 2.8±0.2 vs. 1.9±0.2 ng/ml) respectively. FFR expressed reduced (P<0.05) renal vascular responses to noradrenaline, phenylephrine, methoxamine and Ang II by 36%, 26%, 41% and 56% respectively. The response to Ang II was attenuated by 5‐methylurapidil, CEC and BMY 7378 in the FFR and control, while the adrenergic responses were blunted by 5‐methylurapidil and enhanced by CEC and BMY 7378 in the FFR. These findings suggest that α 1A ‐adrenoceptor is the functional subtype that mediates renal vasoconstriction response in FFR.