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Rank product analysis of gene expression in the medullary thick ascending limb of Henle of Dahl salt‐sensitive rats compared to salt‐resistant SS.13BN consomic rats during the development of salt‐sensitive hypertension
Author(s) -
Yang Chun,
O'Connor Paul,
Liang Mingyu,
Cowley Allen W.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.662.3
Subject(s) - salt (chemistry) , medullary cavity , product (mathematics) , gene , medicine , anatomy , biology , chemistry , endocrinology , genetics , mathematics , geometry
The renal medulla is known to play a fundamental role in the long term regulation of blood pressure in part through the interaction of tubules and vasculature in this region. The current study is the first demonstration in the Dahl salt‐sensitive rat (SS) of the gene expression profile by microarray of a single cell type, the medullary thick ascending limb (mTAL). mTALs were isolated from the outer medulla of both kidneys of SS (n=4) and salt‐resistant SS.13 BN (n=4) (Brown Norway chr 13 introgressed on SS background) rats fed either 0.4%(LS) from weaning or 8%(HS) salt diet for 7 days. A correlation coefficient of 0.988 (n=8 HS microarray chips) confirmed the reproducibility. We found 120 and 82 differentially expressed (DE) genes between strains on HS and LS by using rank product statistical analysis method (FDR<1% and log 2 R>0.5 or <−0.5). The DE genes are enriched on chr 13 from an average of 1.2% present on all chromosomes vs. 5.6% present on chr 13 alone on HS and 1.2% vs 9.1% on LS. Most of the DE genes on chr 13 are localized in the blood pressure QTL 220 for both salt conditions (HS 19 of 20; LS 15 of 22). These data demonstrate the feasibility of carrying out genome wide transcriptional analysis in isolated mTAL to distinguish between strains. Also, the importance of mTAL and genetic factors in salt‐induced hypertension is supported with strong data provided for further molecular pathway analysis. (HL82798)