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Dipropionylcysteine ethyl ester treatment improved recovery after transient focal cerebral ischemia in rat
Author(s) -
Xu Kui,
Kuang Youzhi,
Huang Pengjing,
LaManna Joseph C,
Puchwicz Michelle A
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.650.8
In this study we investigated the effect of the odd‐chain keto‐acid ester, N, S‐dipropionyl cysteine ethyl ester (DPNCE) on recovery following transient focal cerebral ischemia using a rat model of reversible middle cerebral artery occlusion (MCAO). Male Wistar rats (2–3 month old, 250–300g) underwent reversible middle cerebral artery occlusion (MCAO; 2 hr occlusion). DPNCE (450 μmol/kg/min) was administered during the first 30 minutes of reperfusion in the treatment group; saline was given to the control group instead. Behavioral performance (an inclined‐screen test and an adhesion removal test) and infarct volume were determined 7 days following reversible MCAO. The infarct volume in the DPNCE‐treated group was significantly lower compared to the control group (30.4 ± 6.7; n=6 vs. 50.7 ± 5.6; n=7, mean ± SD % contralateral hemisphere, respectively; p < 0.05, t‐test). Behavioral performance was also improved in the DPNCE‐treated group, represented by higher success rate in the inclined screen test and adhesion removal test. Our data showed that DPNCE resulted in improved outcome following an ischemic insult. These data support the concept that neuroprotection and improved outcome can be obtained through stabilization of energy transfer. Treatment with DPNCE during recovery from middle cerebral artery occlusion/reperfusion shows promise as a potential pharmacological agent post‐stroke.