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Involvement of Metabotropic Glutamate Receptor mGlu5 in Pudendal Inhibition of Bladder Overactivity in Cats
Author(s) -
Larson Jeffrey,
Shen Bing,
Wang Jicheng,
Roppolo James,
Degroat William C,
Tai Changfeng
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.648.9
This study investigated in cats the involvement of metabotropic glutamate receptor mGlu5 in pudendal inhibition of bladder overactivity. 0.25% acetic acid was used to irritate the bladder and induce bladder overactivity that significantly reduced bladder capacity compared to saline infusion. Pudendal nerve stimulation at a stimulation intensity 3–4 times the threshold (T) for completely inhibiting isovolumetric rhythmic bladder contractions inhibited the irritation induced bladder overactivity and restored bladder capacity to the volume comparable to the saline control capacity. The effect of pudendal inhibition is dependent on stimulation intensity. MTEP, a metabotropic glutamate receptor mGlu5 antagonist at doses greater than 1 mg/kg completely eliminated the inhibition of bladder overactivity induced by low intensity (1T) pudendal nerve stimulation, but did not antagonize the inhibitory effect of higher intensity (3–4T) pudendal nerve stimulation. In the absence of pudendal nerve stimulation MTEP did not change the small bladder capacity caused by AA irritation. These results indicate that mGlu5 metabotropic glutamate receptors are involved in the bladder antinociception induced by pudendal nerve stimulation. This study is supported by the NIH under grants DK‐068566, DK‐090006, DK‐077783, and by the Christopher and Dana Reeve Foundation.