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INCREASED SALT CONCENTRATION IN THE CULTURE MEDIA INDUCES REDOX IMBALANCE IN MACROPHAGE CELLS
Author(s) -
Islam Mohammed T.,
Agarwal Deepmala,
Francis Joseph,
Majid Dewan S.A.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.643.21
An imbalance between nitric oxide (NO) and superoxide (O 2 − ) production is linked to the development of salt‐sensitive hypertension (SSH). Recent studies implicated a role for the mononuclear phagocyte cells that can provide a buffering mechanism for SSH. To examine whether high salt (HS) induces imbalance in NO and O 2 − production in the phagocyte cells, cultured macrophage cells were incubated for 24 hours in control media (110 mM NaCl) and at media containing higher NaCl conc (130 mM and 150 mM). O 2 − activity was determined in these incubated cells by Lucigenin‐chemiluminescence assay and nitrate/nitrite concentration (NO 3 /NO 2 ) was measured using a colorimetric assay. The mRNA levels of eNOS, iNOS and Nox2 in cell extract was measured by real‐time RT‐PCR. O 2 − activity was found to be higher (P<0.001) in cells incubated in media with 130 & 150 mM NaCl (1029 ± 20 and 929 ± 45 RLU/ 100 μL media) when compared to the cell in the control media (361 ± 15 RLU/ 100 μL media) (n=6 in each case). NO 3 /NO 2 in the condition media was higher at 130 mM NaCl (77.6 ± 11.4 μM; P<0.01) but not at 150 mM NaCl (65.8 ± 3.3 μM) compared to that at control media (59.6 ± 5.5 mM). The mRNA level of iNOS was higher but both eNOS and Nox2 mRNA were down regulated in cells at 130 & 150 mM NaCl compared to cells at control media. These data suggest that the HS induces redox imbalance in phagocyte cells that could play a critical role in the development of SSH.