Effects of Nitric Oxide‐Active Botanicals on Biomarkers of Cardiovascular Risks: A Proof of Concept in Humans

Objective Test whether a novel dietary supplement containing nitric oxide active botanicals can modify cardiovascular risk factors in patients over 40. The trial was conducted at the Houston Institute for Clinical Research in Houston, TX. Inclusion criteria for this double blinded, placebo controlled study were subjects over 40 years old with 3 or more cardiovascular risk factors. Median patient age was 56, 63% male and 37% female. After a baseline blood draw, subjects were instructed to take either Neo40 Daily® or placebo 2X/day on an empty stomach for 30 days. Patients were randomized using a randomization table to receive Neo40 Daily® or placebo. Subjects returned for a second blood draw after 30 days. Patients taking Neo40 Daily had a significant increase in both plasma nitrite (p<0.01) and nitrate (p <0.0001) indicating an increase in systemic nitric oxide availability. There was a statistically significant reduction in 72% of patients with elevated triglycerides (>150mg/dL) compared to their starting levels (168±17 mg/dL vs 232±19 mg/dL; p = 0.02). We conclude that combining nitric oxide‐active natural products/botanicals in a supplement shows promise in restoring NO homeostasis in human subjects at risk for cardiovascular disease.