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Effect of malignant mammary MDA‐MB‐231 cell adhesion on microvessel permeability
Author(s) -
Fu Bingmei M,
Cai Bin,
Zeng Min
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.633.7
To investigate the effect of tumor cell adhesion on microvessel permeability in intact microvessels, we measured the adhesion rate of human mammary carcinoma MDA‐MB‐231, and the hydraulic conductivity (Lp) and permeability to albumin (P) of the microvessels at the initiation of tumor cell adhesion and after ~45min cell perfusion in the post‐capillary venules of rat mesentery in vivo. Rats (SD, 250–300g) were anesthetized with pentobarbital sodium given subcutaneously and kept warm on a heating pad. A midline incision (~2inch) was made in the abdominal wall and the mesentery was gently taken out from the abdominal cavity and arranged on the surface of a glass coverslip for the measurement. Individual microvessel was perfused with cells (~4 million/ml) at a rate of ~1000μm/s. At the initiation of cell adhesion, which is defined as one adherent cell in 100μm vessel segment, Lp was 1.7 (±0.3 SD, n=8)‐fold, and P was 2.4 (±1.1 SD, n=8)‐fold of their controls; after ~45min perfusion, the adhesion increased to ~5 adherent cells per 100μm vessel segment, while Lp was 2.9 (±0.6 SD, n=11)‐fold, and P was 5.8 (±2.2 SD, n=10)‐fold of their controls, respectively. Combining these measured data with a mathematical model for the inter‐endothelial transport suggests that tumor cell adhesion to the microvessel wall degrade the endothelial surface glycocalyx. Supported by NIH U54CA137788‐01,SC1CA153325‐01A1 and NSF‐CBET0754158.