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Rat mesenteric lymphatics undergo remodeling and have altered contractility and calcium sensitivity in a model of metabolic syndrome
Author(s) -
Zawieja Scott D,
Wang Wei,
Nepiyushchikh Zhanna,
Wu Xin,
Zawieja David C,
Muthuchamy Mariappan
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.632.2
Metabolic Syndrome (MS) is a cluster of metabolic abnormalities that results in a profound impact on cardiovascular health and immune function. Only few studies have studied the impact of MS on lymphatic function, which is a nexus between the vascular system and the immune response. Since mesenteric lymphatics (ML) operate in a chronically inflamed microenvironment under MS, and are the highways for the transport of chylomicrons and antigen presenting cells, we hypothesized that MS results in ML remodeling and alterations in ML function. Rats were fed a 60% fructose diet for 7 weeks to produce a model of MS. We utilized a cannulated/pressurized isolated vessel approach and found that ML diameters were smaller (p=.06), contraction frequency lower (p<.05), and that a compensatory increase in stroke volume (SV) (p<.05) failed to maintain the effective lymph pump flow (LPF) (p<.05) in MS as compared to control. Furthermore, these contractile changes persisted under enforced flows (Frequency p<.05, SV p<.05, LPF p<.05). Wire myograph studies of permeabilized MLs showed a decreased Ca sensitivity (pCa 50 5.83±.08 vs. 5.54±.06,p<.05) and half the maximal force production (0.11mN/mm vs. 0.26 mN/mm ,p<.05) in MS MLs compared to controls. Thus we conclude that MS resulted in ML remodeling and impaired the intrinsic flow producing contractile property of MLs. Supported by NIH‐HL070308, HL80526 and HL86650.