Pharmacological Assessment of a Hialuronan Jelly to Wound Healing. Biochemical Evaluation of Dermal reconstitution

To assess the dermal reconstitution in open wounds treated with Hyaluronan jelly (HA) biochemical indicators such as prolin, hidroxyprolin and total amino acid content in dermis were determined. Methods Jelly containing 0 (placebo), 2, 4 and 8% HA were applied to excisional wound in the similar area of dermis of rat skin and their effect assessed on 5th and 7th days. Acid hydrolysis were made with the objective of quantified the alpha amino acid groups from nitrogen content and total amino acid with. Results The values of alpha amino nitrogen were the highest for the 4% jelly on the 7th day with 4, 32 mg.area‐1, followed by concentration to 8 %. Glycine was the most abundant on day 7 with 310 and 402 ìg.area‐1 in the jelly to 4 and 8 % with a hydroxyproline of 10, 3 and 13, 3 ìg.area‐1 this is a direct relationship between the behaviour of the two treatments and two assays it coincides with histology matrix results in collagen fibres of the jelly to 4 % obtained a 100 % response were obtained. The alpha amino nitrogen and glycine quantitative values indicate collagen synthesis and dermal reconstitution, the quantitative hydroxyproline value shows adequate response, because this amino acid is a marker biochemical for this. Correlation between the biochemical and histological results in the dermis indicate that the 4% HA is the most effective among the examined formulations in terms of dermal reconstitution.