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Subcutaneous tissue response of isogenic mice to a triantibiotic paste used as intracanal medication
Author(s) -
Faria Gisele,
Pereira Maristela Soares Swerts,
Silva Lea Assed Bezerra,
TanomaruFilho Mario,
GuerreiroTanomaru Juliane Maria,
Kuga Milton Carlos,
Rossi Marcos Antonio
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.612.1
The use of mixture of antibiotics to treat immature teeth with apical lesion has been proposed to achieve revascularization of root canal. The aim of this study was evaluated the response of subcutaneous connective tissue to a triantibiotic paste (TP) consisting of metronidazole, ciprofloxacin and minocycline compared to calcium hydroxide. Thirty mice were assigned to 2 groups. Each animal received 2 polyethylene tube implants as follows: Group I (n=30 tubes) ‐ calcium hydroxide paste (Calen) and Group II (n=30) ‐ TP for 7, 21, and 63 days. The tissues were submitted to histological processing and analyzed using a five‐point scoring system considering the collagen fiber formation, reaction tissue thickness, inflammatory infiltrate and number of vessels. Group I showed areas of discrete fibrosis, normal conjunctive tissue, small amount of blood vessels and few inflammatory cells. Group II induced an exuberant inflammatory reaction, and formation of a large amount of granulation tissue showing many blood vessels, fibroblasts, inflammatory cells even 63 days after implant placement presenting statistically significant difference (Mann‐Whitney test, p>0.05) from the group I. In conclusion, the Calen paste allowed an adequate tissue response, whereas the TP induced the formation of large amount of granulation tissue which could be responsible for root canal revascularization. Supported by PROPe‐UNESP.