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Metabolic studies of pyridoxamine (Vitamin B6) metabolism in rats
Author(s) -
Ericson Karen Louise,
Coburn Stephen P.,
Gallant Maxime A.,
Reinwald Susan,
Burr David B.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.608.3
Subject(s) - pyridoxamine , vitamin b , metabolism , chemistry , vitamin b6 , vitamin , biochemistry
Pyridoxamine (PM), a metabolic isomer of vitamin B 6 , has been studied as an agent to reduce/prevent diabetic complications, based upon its ability to prevent formation of advanced glycation end‐products and act as an anti‐oxidant (Voziyan et al., Cell. Mol. Life Sci. 62:1671,2005). As a preliminary study determining effect of PM on bone collagen matrix in diabetic rats, Sprague‐Dawley rats were given subcutaneous saline (control), 50 mg/kg, or 100 mg/kg pyridoxamine; plasma and urine samples were collected over 24 hours. Samples were analyzed by HPLC to quantitate vitamers of vitamin B 6 (PLP, 4PA, PL), as well as PM and pyridoxamine‐5′‐phosphate. Urine samples were also analyzed for 4PA, and also PM and N‐methylpyridoxamine, to determine if rats, like dogs, are able to methylate pyridoxamine (Ericson et al., Bioorg.Med.Chem.Lett. 18:1845 2008). Data were analyzed using the WINSAAM modeling program, and the plasma half life of PM in rats receiving 50 mg/kg was calculated at 1.43 hours; the half‐life in rats receiving 100 mg/kg was calculated to be 1.6 hrs. Supported by NIH grant R01 AR 047838 (DBB).