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Iron bioavailability in lentil based diets: Studies in poultry and in vitro digestion/Caco‐2 model
Author(s) -
Tako Elad,
Vandenberg Albert,
Thavarajah Dil,
Thavarajah Pushparajah,
Glahn Raymond P
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.607.8
Subject(s) - bioavailability , biofortification , digestion (alchemy) , food science , chemistry , crop , in vitro , agronomy , biology , micronutrient , biochemistry , chromatography , bioinformatics , organic chemistry
Lentils (Lens culinaris) are a pulse crop that is relatively high in Fe concentration (50–100 ppm) and consumed worldwide. As such lentils are an excellent candidate for Fe biofortification. We measured Fe bioavailability and Fe concentration of 20 commercial lines of lentils from a major lentil production region in Saskatchewan, Canada. Using an in vitro digestion/Caco‐2 cell model for Fe bioavailability screening, we found that significant differences in Fe bioavailability exist within the commercial lines. Fe concentration was also found to differ, ranging from 55–95 ppm. Therefore, we selected three lentil varieties to determine if the high‐Fe lentils would improve the Fe status of our animal model (broiler chickens) when fed over a 30 day period. We formulated diets containing 50% lentils, broiler hatchlings were divided into three groups (n=10), and were fed the following: lentil 1 (“Red berry”,95ppm Fe), lentil 2 (“Red berry”,75ppm Fe) or lentil 3 (“Milestone”,55ppm Fe). Diets had no supplemental Fe and were differerent in Fe content (65, 55, 45 ppm Fe, respectively). For 4 wks, hemoglobin (Hb), feed consumption and body weights were measured. Results showed higher hemoglobin values and total body Hb‐Fe values in “Lentil 1” group vs. “Lentil 3” (p<0.05). We conclude that the in vivo results support the in vitro observations, ie. the 40ppm difference in lentil Fe provides significantly more bioavailable Fe.