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Characteristics of the diet and iron overload risk in a group of healthy adult males from Argentina
Author(s) -
Castro Marcelo,
Rey Jorge A,
Lardo Marta M,
Ceballo María F,
Lazarowski Alberto,
Langini Silvia H
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.607.7
Feeding habits in Argentina are characterized by high bovine meat intake‐ (54.9 kg/per capita/year, FAO 2007). However wheat flour fortification with iron (Fe) is mandatory since 2002. Therefore, because of Fe overload risk, transferrin saturation (TS) and HFE mutations were studied in a group of healthy adults. Samples negative for infectious diseases and C‐reactive protein (PCR‐latex, Wiener lab) were obtained from 111 male blood donors (19–72 y) attending the Blood Bank at the Hospital de Clínicas, University of Buenos Aires, Argentina, during 2007 and 2008. Serum Fe (sFe) (FerroZine) was measured on an automated clinical analyzer (Hitachi 917, Roche). The total iron binding capacity (TIBC) was calculated, and the percentage TS as the sFe/TIBC × 100. Genotyping for the C282Y, H63D and S65C mutations in the HFE gene was performed by PCR‐RFLP in blood samples collected with EDTA or spotted on filter paper. HFE genotypes and TS were:Genotype % of donors % of donors with TS ≥ 50%100 9 H63D 20.7 (23/111) 21.7 (5/23) S65C 3.6 (4/111) 0 (0/4) C282Y 3.6 (4/111) 75 (3/4)Heterozygosity and homozygosity for H63D, S65C and C282Y mutations were: 82.6% and 17.4%, 100% and 0%, 75% and 25%, respectively. Two donors were compound heterozygotes for H63D/S65C and H63D/C282Y, respectively. These preliminary results alert about potential adverse effects of excessive oral Fe intake in argentine healthy people at risk of genetic haemochromatosis. Supported by UBACyT 20020090200114, Argentina.