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Alleviation of bowel inflammation by dietary phenethylisothiocyanate in a mouse ulcerative colitis model
Author(s) -
DEY MOUL,
LIU YI,
REUHL KENNETH,
RASKIN ILYA,
RIBNICKY DAVID,
KUHN PETER
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.599.6
Phenethylisothiocyanate (PEITC) produced by Brassica food plants is known for various health promoting properties but its effect in alleviation of ulcerative colitis signs was unknown. In acute and chronic efficacy studies oral administration of PEO ( P EITC E ssential O il) was effective at remitting signs of ulcerative colitis (UC) in mice. Disease activity, histology and biochemical characteristics were measured. PEO treatment improved body weights, stool consistency and reduced intestinal bleeding, mucosal inflammation, depletion of goblet cells and infiltration of inflammatory cells and proinflammatory interleukin1β in the colons. Expression analyses were carried out for immune function related genes, transcription factors and cytokines in lipopolysaccharide‐activated mouse macrophage cells. PEO affects an intricate network of immune signaling genes including a novel concentration dependent reduction of total cellular Signal Transducer and Activator of Transcription 1 (STAT1) as well as nuclear phosphorylated‐STAT1 (activated form). Decreased mRNA expression of C‐X‐C motif ligand 10 (a STAT1 responsive chemokine) and Interleukin 6 were also observed. Biological activities of PEO could be associated with suppression of activation of STAT1 transcription and inhibition of pro‐inflammatory cytokines. Research funding from National Institutes of Health, Bethesda, MD [4R00AT00424503]