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Gene expression and pathway changes after n‐3 PUFA supplementation in healthy subjects
Author(s) -
Rudkowska Iwona,
Paradis AnnMarie,
Thifault Elisabeth,
Julien Pierre,
Couture Patrick,
Lemieux Simone,
Vohl MarieClaude
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.599.4
Gene expression profiling is thought to be more sensitive to nutritional intervention than the traditional biochemical parameters. Objective To investigate the changes in gene expression in peripheral blood mononuclear cells (PBMCs) and the associated changes in metabolic pathways following n‐3 polyunsaturated fatty acid (PUFA) supplementation. Methods Transcriptome analysis of a 6‐week supplementation with n‐3 PUFA (3 g/d) in PBMCs of 29 healthy men and women was carried out. Changes in gene expression were assessed by the Human HT‐12v4 Expression BeadChips (Illumina). Results After supplementation, erythrocyte n‐3 PUFA concentrations increased and plasma triglycerides decreased significantly to a similar extent in both genders. Data demonstrate that among the 47,231 transcripts present on microarray, approximately 69% of transcripts were detected in PBMCs of subjects. N‐3 PUFA supplementation changed the expression of 610 genes in men, whereas the expression of only 250 genes was altered in women. Pathway analyses indicate changes in gene expressions via the nuclear receptor peroxisome proliferator‐activated receptor alpha ( PPARA ) and PPARA/Retinoic X Receptor A ( RXRA ) activation. Conclusion These results indicate that n‐3 PUFA supplementation can alter favourably gene expression profiles and related pathways in PBMCs. Funding: CIHR.