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Effects of arsenic exposure and folate deficiency on methyl metabolism in human fibroblast cell lines
Author(s) -
Chango Abalo,
Bousserouel Souad,
Gnimpieba Etienne Z,
Nour Afif M Abdel,
AbdennebiNajar Latifa
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.598.2
We evaluated the impact of arsenic (As) on methylation process pathways in human skin fibroblast cell line in folate depleted medium (FDM) or in experimental control medium (ECM). We observed regional hyper‐ and hypomethylation in genomic DNA. hrfc1 transcript level in FDM was decreased significantly than in ECM. The fold change for DNA‐methyltransferase dnmt1, expression was significantly increased in FDM than in ECM and dnmt3a was significantly highly expressed in ECM than in FDM. Study showed significant increase of micronuclei in cells grown in FDM than in ECM. Results may suggest differences in maintaining methylation and de novo methyation, and chromosomal instability secondary to the alteration in the dUMP methylation pathway.