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Persistence of Lactobacillus Reuteri DSM17938 in the Human Intestinal Tract in Response to Different Storage Conditions
Author(s) -
Smith Tracey J,
Anderson Danielle J.,
Sikes Anthony,
Margolis Lee M.,
Young Andrew J.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.584.15
Subject(s) - lactobacillus reuteri , probiotic , persistence (discontinuity) , gastrointestinal tract , feces , food science , volunteer , medicine , biology , bacteria , microbiology and biotechnology , chemistry , ecology , genetics , geotechnical engineering , engineering
Probiotic‐containing foods are consumed to enhance gastrointestinal (GI) and immune function. How storage of those foods affects persistence of probiotics in the GI tract are undefined. We evaluated persistence of Lactobacillus reuteri DSM17938 ( L. reuteri; BioGaia, Stockholm, Sweden) in the human GI tract after consuming “fresh” (FRESH; n = 9; room temperature ≤ 1 week followed by freezer) or “stored” (STORED; n = 10; 37°C for ~7 days followed by freezer) L. reuteri ‐containing pudding (10 8 and 10 9 CFU/serving, respectively) for 7 days. Fecal samples were collected daily during probiotic supplementation (D1–7) and intermittently after probiotics were discontinued (D13–15 and D20–22), and analyzed for L. reuteri . Results are reported in 3‐day increments (D2–4, D5–7, D13–15, and D20–22). All volunteers in FRESH, and 1 volunteer in STORED, had detectable L. reuteri during supplementation (D1–7). L. reuteri count rose in FRESH ([mean ± SD] D2–4: 4 x 10 4 ± 2 x 10 4 CFU, p < 0.01; D5–7: 10 x 10 4 ± 9 x 10 4 CFU, p < 0.01), but not in STORED (D2–4: 3 x 10 2 ± 9 x 10 2 CFU, P = 0.3; D5–7: 1 x 10 2 ± 4 x 10 2 CFU, P = 0.3). More volunteers had detectable L. reuteri 1 and 2 weeks after supplementation ended in FRESH (4/9 and 2/9, respectively) compared to STORED (0/10 and 0/10, respectively). Colonization of L. reuteri is achievable with daily intake of “fresh”, but not “stored” L. reuteri . Funded by the Medical Research and Materiel Command.