Premium
Effects of short term and long term consumption of soy germ and/or tomato powder on expression and activity of hepatic phase I and phase II enzymes in rats
Author(s) -
Zuniga Krystle Erin,
Erdman John W
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.584.12
Subject(s) - bioavailability , carotenoid , chemistry , enzyme , medicine , cytochrome p450 , endocrinology , cyp1a2 , enzyme assay , in vivo , biochemistry , food science , pharmacology , biology , microbiology and biotechnology
In vitro and in vivo evidence suggest that isoflavones and carotenoids may modulate the activity and expression of phase I and phase II enzymes, which may be a mechanism by which soy or tomato consumption can reduce the risk of chronic disease. Diets containing 10% tomato powder (TP), 2% soy germ (SG), neither, or a combination (TP+SG) were consumed by 7‐week‐old or 34‐week‐old male Copenhagen rats for 25 weeks or 7 days, respectively. We previously reported that in the 7‐day study, serum carotenoids were significantly lower in TP+SG compared to TP alone, and long term TP+SG consumption resulted in reduced carotenoid bioaccumulation and increased urinary isoflavone excretion. To determine if consumption of TP or SG alters detoxification enzymes and if the differences in bioactive bioavailability affect bioactivity, hepatic mRNA expression and activity of select phase I and phase II enzymes were measured. Expression and activity of NAD(P)H quinone oxidoreductase 1 (NQO1) was not significantly altered by diet in the 25 week study, and diet had no effect on NQO1 activity in the 7‐day study. Activity of hepatic cytochrome P450 1A, as measured by ethoxyresorufin O‐deethylase, and mRNA expression of CYP1A1 and CYP1A2 will be reported. Our results suggest that consumption of tomato and/or soy germ has little effect on activity or expression of hepatic phase II detoxification enzymes. Funded in part by USDA NNF 2005‐03750. Grant Funding Source : USDA NNF 2005‐03750