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Effect of blueberry polyphenols on 3T3‐F442A preadipocyte differentiation
Author(s) -
Moghe Shiwani,
Juma Shanil,
Vijayagopal Parakat
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.583.7
The prevalence of obesity has increased world‐wide. Plant polyphenols have been shown to combat adipogenesis and induce lipolysis. However, very little is known regarding the role of blueberry polyphenols on adipocyte differentiation. Hence we tested the dose‐dependent effects of blueberry polyphenols on mouse 3T3‐F442A preadipocyte differentiation and lipolysis. 3T3‐F442A preadipocytes were incubated with three doses of blueberry polyphenols (BB‐150, BB‐200 and BB‐250 μg/mL) and intracellular lipid content, cell proliferation and lipolysis were assayed. Blueberry polyphenols showed a dose‐dependent suppression of adipocyte differentiation determined by oil red‐O staining and adipored assay. Intracellular lipid content in control (11,385.51 ± 1169.6, relative fluorescence units) was significantly higher (P < 0.05) than the three doses of blueberry polyphenols (8336.86 ± 503.57), (4235.67 ± 323.17), and (3027.97 ± 346.61). This corresponds to a reduction of 27%, 63% and 74%, respectively. Cell proliferation was observed to be significantly high in control (0.744 ± 0.035, optical density units) than BB‐150 (0.517 ± 0.031), BB‐200 (0.491 ±0.023), and BB‐250 (0.455 ± 0.012). However, when tested for lipolysis, there was no significant difference observed among the groups. We conclude that blueberry polyphenols may play an effective role in inhibiting adipogenesis and cell proliferation.