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Correlation of iNOS, eNOS, PPARγ, TNFα and HNF4α gene expressions in subcutaneous adipose tissue of obese women in response to 12‐week calorie‐restricted diet with or without exercise
Author(s) -
Barrera Eliud Salvador Aguilar,
Avendaño Jazmín Iveth Alfaro,
López Aarón Domínguez,
Angeles Martínez Godinez María,
López Modesto Gómez,
García Giorgio Daniel Ibarra,
PerezVielma Nadia,
Padilla Eleazar Lara,
García Angel Miliar
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.582.14
OBJECTIVE Determine the effects of weight reduction by a 12‐week calorie‐ restricted diet with or without exercise on iNOS, eNOS, PPARγ, TNFα and HNF4α gene expressions in subcutaneous adipose tissue of obese women. RESEARCH DESIGN AND METHODS Twelve women volunteers with BMI >30 kg/m 2 participated in 2 separate experimental trials. The trials differed only by whether the women performed diet (D) or diet plus exercise (DE). In the DE, aerobic exercise was performed 3 days per week (30′/session). Biopsy of subcutaneous adipose tissue before and after the program was processed by Real Time‐PCR for all genes. RESULTS The relative expression of mRNA by iNOS, eNOS and PPARγ genes showed a significant decrease in the D, p=0.028, p=0.046 y p=0.028 respectively. A decrease in the iNOS (p=0.028) and PPARγ (0.075) gene expressions was observed in the DE. TNFα and HNF4α genes was not significant differences. We found a positive correlation between the iNOS and eNOS gene expressions and the BMI (SC=0.771) for both groups. CONCLUSIONS A caloric restricted diet (D) and (DE) have an influence on iNOS and eNOS gene expressions. The positive correlation between PPARγ and TNFα mRNA expression shows that these transcriptional factors may regulate the NOS expression in these women. These genes could be therapeutic targets for new drugs in obesity treatment.