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Phenolic Phytochemicals and Seed Oil Characterization of Ziziphus Mauritiana L. Fruit Grown in Pakistan
Author(s) -
Memon Ayaz,
Luthria Devanand,
Memon Najma,
Bhanger Muhammad
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.581.7
Subject(s) - ziziphus , chemistry , gallic acid , food science , mauritiana , antioxidant , botany , biology , organic chemistry
Ber is a tropical fruit which grows from the tree species, Ziziphus mauritiana Lamk. The pericarp of this fruit is consumed either fresh or dried while its seeds are usually discarded as waste. The present study evaluates the antioxidant activity and phenolic content of the fruit. The edible portion of the fruit was extracted with 60% aqueous methanol by sonication and then assayed for total phenolic content, antioxidant activity, and individual phenolic compounds by HPLC‐DAD. The total phenolic content of the fruit was found to be 1.28 ¡Ó 0 .01 g/100g gallic acid equivalent, with an antioxidant activity of 50.40 ¡Ó 2 .04ƒÝmol/100g. Hydroxybenzoic acid, vanillin, ortho‐ and para‐coumaric acid, epicatechin, querectin, and naringenin were identified by matching retention time and UV spectra with those of commercial reference standards. Comparison and optimization of extraction and base hydrolysis techniques revealed that sample preparation plays a significant role in quantification of phenolic phytochemicals extracted from the Ber samples. The seed of Z. mauritiana L. contained 38% oil that is rich in unsaturated fat (7‐octadecenoic acid) with ƒ×‐tocopherol and stigmasterol as value‐added compounds. This research was supported by the Agriculture Research Service, USDA and the Higher Education Commission, Pakistan. Grant Funding Source : USDA and Higher Education Commission