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Feasibility of nutrient photoprotection studies in a three‐dimensional human skin tissue model: Using alpha tocopherol
Author(s) -
Evans Julie A,
Chen CY. Oliver,
Garlick Jonathan,
Wang XiangDong,
Johnson Elizabeth J
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.581.12
Subject(s) - photoprotection , tocopherol , alpha tocopherol , nutrient , alpha (finance) , vitamin e , medicine , biology , antioxidant , surgery , ecology , biochemistry , photosynthesis , construct validity , patient satisfaction
Alpha‐tocopherol (AT) has previously shown promise as a photoprotectant. A 3‐dimensinal (3‐D) human skin tissue model may provide a useful tool for studying AT photoprotection. This study aimed to determine the feasibility of AT uptake in a 3D skin model with ultraviolet A (UVA) irradiation. AT was applied to media (0 – 50 μM) or topically (0 – 25 mg/cm 2 ) for up to 48 h. Skin absorption of AT was quantified in dermis and epidermis by RP‐HPLC with UV detection after proteolysis and standard lipid extraction. Tissue was exposed to ultraviolet A (UVA) irradiation at 0 – 100 J/cm 2 and morphology (from hematoxylin & eosin stain) and apoptosis (by TUNEL assay) were assessed after 48 – 96 h. Skin AT content increased in a dose and time‐dependent manner. After 48 h media incubation with 25 μM AT, dermal and epidermal concentrations of AT were 0.2 and 0.5 ng/mm 2 , respectively, comparable to those found in human skin. After 2 h incubation with 1 mg/cm 2 topical AT, dermal and epidermal concentrations of AT were 45 and 378 ng/mm 2 , respectively. In a dose‐dependent, reproducible manner, UVA induced apoptosis and morphology changes. In conclusion, AT is absorbed into both compartments of this skin model from media and topical application, which provides a useful model for studies of AT and skin health. The assessment of photoprotection provided by AT in the 3‐D skin model is underway. Support: USDA#581950‐7‐07; Almond Board of California