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Characterization of rabbit mesenchymal stem cells
Author(s) -
Yaszemski Alexandra,
Rooney Gemma E,
Windebank Anthony J
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.559.9
Mesenchymal stem cells (MSCs) are multipotent stem cells that can be isolated from bone marrow and adipose tissue. They are characterized by their adherence to plastic and ability to differentiate into adipocytes, osteocytes and chondrocytes. The objective of this project was to thoroughly characterize rabbit MSCs isolated from adipose tissue. Few markers have been identified for characterizing rabbit MSCs. This is due, in part, to the availability of rabbit antibodies that cross react with the rabbit cells. Protocols for differentiating rabbit MSCs into mesodermal cell types have also yet to be optimized. FACS analysis and immunofluorescent microscopy of FITC labeled immuno‐stained markers was performed. Protocols for differentiation into adipocytes, osteocytes and chondrocytes were adapted from other species. Variation in treatment included addition of FBS, platelet lysate, or selected growth factors. Rabbit MSCs were found to express CD44, CD120a, CD71 and Vimentin, with only approximately 20% of cells expressing CD90. Differentiation into adipocytes was successful in the presence of FBS. Chondrogenic and osteogenic differentiation protocols were unsuccessful. This research has identified several markers, which can be used to characterize rabbit MSCs. In order to establish a more complete characterization profile, differentiation into osteocytes and chondrocytes will have to be further investigated and optimized.