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Effects of lithium chloride and valproic acid on phospholipid composition of Tetrahymena patula
Author(s) -
Lawrence An Lam,
Ryals Phillip E
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.559.3
Subject(s) - chemistry , tetrahymena , phospholipid , lithium chloride , composition (language) , lithium (medication) , valproic acid , food science , biochemistry , biology , organic chemistry , endocrinology , linguistics , philosophy , neuroscience , membrane , epilepsy
Morphological changes occur in Tetrahymena patula that affect oral apparatus structure in response to lithium chloride (LiCl) and valproic acid (VPA). Both are common treatments of bipolar disorder and have been shown by others to alter phospholipid composition in human neuroblastoma cells and in murine models; however, little is known about the specific mechanisms involved. T. patula dedifferentiate from a macrostomal to microstomal state in response to 20 mM LiCl and 250 μM VPA treatments. To ascertain the effects on percent phospholipid class composition, polar lipids were separated using silica gel thin‐layer chromatography in a chloroform/acetic acid/methanol/water (75:25:5:2.2; v/v) mobile phase. Lipid phosphorus content was determined by phosphorus assay. The percentage of aminoethylphosphonolipid increased in both LiCl and VPA (26.5% and 33.2%, respectively) compared to the control (21.6%). In additional experiments, total phospholipid was separated using Jolles solvent to resolve phosphoinositides. Preliminary densitometric analysis showed consistent percentages of phosphoinositides between controls and treatments. Data suggest that both LiCl and VPA alter phospholipid composition in T. patula .