Influence of copper in the pattern of genes involved in the interaction of Paracoccidioides brasiliensis with the extracellular matrix component

Paracoccidioides brasiliensis (Pb) is a fungus agent of paracoccidioidomycosis and the strategies used by the pathogen may be the expression of proteins related to adaptation to the host conditions, related to micronutrients uptake and it's capacity to provoke human illness depends on factors like virulence and interaction with the host structures like the extracellular matrix components (ECM) that plays an important role in the regulation of cell adhesion. This study proposes an analysis of transcripts expressed when depleted of copper in the presence of ECM ‐ laminin, fibronectin and type I and IV collagen by Representational Difference Analysis (RDA). We cultured the Pb in a chemically defined media without copper (Cu) and them the fungus was placed in contact with the four ECM. RNA was obtained of Pb without Cu (driver), and Pb without Cu in contact with the ECM (tester) and RDA assay was developed. Two hybridizations were performed, with an excess of driver to remove the common sequences. The differentially expressed products revealing 33 genes involved in differents process like virulence, protein synthesis, metabolism, energy, transcription, transport, stress response and cell cycle. These data, it's important for understand the roles of genes role in the pathogenicity and contributes to understand how Pb acts against the depletion of Cu and in conditions that simulating the host environment.