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A proteomic approach to analysis of functional effects of fatty acids on NIH3T3 fibroblasts
Author(s) -
Magdalon Juliana,
Hatanaka Elaine,
Romanatto Talita,
Scaife Caitriona,
Newsholme Philip,
Curi Rui
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.554.3
Knowing that fatty acids influence fibroblast function, the aim of this study was to assess the effect of treatment with oleic, linoleic or palmitic acids (50μM) for 24h on protein expression in NIH3T3 fibroblasts by proteomic analysis. The fatty acids were not toxic to the cells according to membrane integrity and DNA fragmentation measurements, although MTT assay showed an increase in absorbance in the treated cells. A proteome analysis was made using two‐dimensional difference gel electrophoresis (2D‐DIGE). Cells treated with oleic, linoleic or palmitic acids showed 24, 22, 16 spots differentially expressed, respectively, and nine of them were identified by mass spectrometry using LC/MS/MS. Among the proteins changed, α‐enolase and far upstream element binding protein 1 are directly involved in many pathologies, since the former activates plasmin and the latter modulates c‐myc expression. Moreover, eukaryotic translational initiation factor 5A‐1, transcription factor BTF3, FK506‐binding protein 4 and heterogeneous nuclear ribonucleoprotein L were also changed. In conclusion, these results suggest that fatty acids may modulate the expression of some important proteins involved in pathologies and thus may be used as therapeutic agents. Support: FAPESP, CNPq and CAPES.