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RNA export factor Rae1 interacts with cohesin subunit SMC1 at the mitotic spindle pole
Author(s) -
Funasaka Tatsuyoshi,
Wong Richard
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.546.1
The faithful segregation of daughter chromosomes during cell division depends on formation of a microtubule‐based bipolar spindle apparatus. We found that the cohesin subunit structural maintenance of chromosomes subunit 1 (SMC1) is recruited to microtubule‐bound RNA export factor 1 (Rae1) at the mitotic spindle pole. We identified a mitotic‐specific interaction between Rae1 and SMC1 and explored the relationship between Rae1 and SMC1 in spindle formation. We located the Rae1‐binding site to SMC1(947‐967) and provided several lines of evidence that phosphorylation of Ser(957) and Ser(966) of SMC1 stimulates binding to Rae1. Imbalances in these assembly pathways caused formation of multipolar spindles. Our data suggest that cohesin's known bundling function for chromatids in mitotic and interphase cells extends to microtubules at the spindle pole.