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Transcriptomic and Histopathologic Characterization of Mouse Model(s) Simulating Features of Post‐Traumatic Stress Disorder
Author(s) -
Muhie Seid,
Hammamieh Rasha,
Miller Stacyann,
Meyerhoff James,
Carroll Erica,
Jett Marti
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.535.2
Social defeat mouse models of post‐traumatic stress disorder (PTSD) were used to simulate human PTSD. C57B6 mice exposed to SJL aggressor mice exhibited PTSD phenotypes: freezed motion, aggressor barrier avoidance, startled jumping, decreased‐object recognition and tail suspension immobility. Hemi‐brain, hippocampus, amygdala, medial prefrontal cortex, blood, spleen and heart of control and defeated C57B6 mice were analyzed using Agilent's genome‐wide (mouse) microarray platform. More than 3000 transcripts were differentially regulated. Differentially regulated transcripts (in brain‐regions) were found to be involved in neural tube formation, neural crest cell differentiation, neurotransmitter secretion, hippocampus development, cerebral cortex GABAergic interneuron migration, learning and memory, oligodendrocyte differentiation, dendrite morphogenesis, and forebrain – midbrain boundary structural organization. Transcripts, differentially regulated in blood, spleen and heart samples, were significantly associated with immune response and metabolic disorders. Histopathology analyses of organs showed inflammation in heart vessels and gall bladder in defeated C57B6 mice. Phenotypic markers, histopathology and gene regulation data showed validity of social defeat mice as human PTSD models in search of early biomarkers of PTSD. Funding: Office of the Army Surgeon Genera