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Evaluation of the effectiveness of the insulin‐mimetics, selenium and vanadium, in insulin‐resistance in primary hepatocytes
Author(s) -
Coulibaly Sylvie,
McPherson Kalan,
Nair Sandhya,
Ruff David,
Stapleton Susan R.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.530.5
Subject(s) - insulin resistance , vanadium , selenium , insulin , medicine , primary (astronomy) , chemistry , endocrinology , inorganic chemistry , physics , organic chemistry , astronomy
Insulin‐mimetics are agents that have been shown to mimic the actions of insulin including promoting the entry of glucose into tissues, activating signal proteins, influencing the expression of genes and regulating metabolic processes. Through the years, selenium and vanadium compounds have been shown to mediate a number of insulin‐like actions in models of Type I diabetes both in vivo and in vitro. Few studies, however have ascertained the effectiveness of these mimetics on models of Type II diabetes or insulin resistance. Using glucosamine, a precursor of the hexosamine biosynthetic pathway (HBP) products, models of insulin resistance in a variety of cells in culture have been established. Previously we have demonstrated insulin resistance in primary rat hepatocytes by treating the cells with glucosamine. Using this model we now investigate the effects of the insulin mimetics, selenium and vanadium on insulin signal proteins and expression of the key metabolic genes, phosphoenolpyruvate carboxykinase, glucose‐6‐phosphate dehydrogenase and fatty acid synthase.