A new look on protein dynamics in yeast

The budding yeast Saccharomyces cerevisiae can maintain homeostasis in a constantly changing environment. Although much effort has been invested to explore transcriptional regulation in yeast it is now becoming evident that many proteins essential for the response to environmental stimuli show no transcriptional response. This would indicate that post‐translational regulation plays an important role. Here we show an accurate and reproducible microscopic platform for measuring protein localization and abundance in yeast in high throughput. Using the commercially available yeast GFP library, we have used our platform to assess protein dynamics under five different conditions. We find that, surprisingly, more than 1% of the proteome changes organelle localization in each condition uncovering an unprecedented number of dual localizations. Moreover, in each condition we characterize a large number of post‐transcriptional regulation events. For easy access to this data we have created a database called LoQAtE (Localization and Quantitation Atlas of yEast proteome). Using the information in LoQAtE we can unravel the general rules guiding proteome localization and abundance changes under diverse cellular and environmental conditions. Moreover, these data provide a new perspective on the richness and diversity of cellular defense mechanisms. This project is supported by Minna James Heinemann grant.