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BMP‐2 induces VEGF expression in cultured human osteoblast: a possible role for reactive oxygen species and HIF‐1α
Author(s) -
Akeel Sara,
ElAwady Ahmed,
ElSalanty Mohammed,
Sharawy Mohamed,
AlShabrawey Mohamed
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.492.2
Subject(s) - reactive oxygen species , osteoblast , vegf receptors , microbiology and biotechnology , chemistry , oxygen , cancer research , biology , biochemistry , in vitro , organic chemistry
Objectives : Bone morphogenetic proteins (BMPs) and vascular endothelial growth factor (VEGF) have been reported to be crucial in endothelial cells/osteoblasts interaction. The relative hypoxia and subsequent production of hypoxia‐inducible factor‐1a in the site of bone injury have been reported to play a role in fracture repair probable via VEGF upregulation. Additionally, our and others reports demonstrated the crucial role of reactive oxygen species (ROS) in signaling VEGF expression. Our aim is to investigate whether ROS and HIF‐1 α‐dependent VEGF expression enhance BMP2‐induced bone formation through modulation of angiogenesis. Methods The effect of BMP‐2 on VEGF and HIF‐1 α expression in normal human osteoblasts NHOst using ELISA, immunofluorescence and RT‐PCR. ROS production was assessed by dihydroethidine staining and lipid peroxidation. Results BMP‐2 increased HIF‐1 α and VEGF mRNA and protein expression in cultured NHOst compared to the control (P<0.05). Furthermore, ROS production significantly increased by BMP‐2 (P<0.05). Conclusion Taken together our data suggest that ROS, HIF‐1 α and VEGF production might be involved in mediating the biological effects of BMP‐2 Grant Funding Source : King Abdulaziz University