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Antiobesity and Antiinflammatory Effects of Lactobacillus sp. OPK‐3 Isolated from Kimchi in 3T3‐L1 Adipocytes, RAW 264.7 Macrophage cell and C57BL/6J mice
Author(s) -
Park JeongEun,
Oh SukHeung,
Kim BumSeok,
Cha YounSoo
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.438.1
Subject(s) - macrophage , 3t3 l1 , chemistry , microbiology and biotechnology , biology , biochemistry , adipocyte , adipose tissue , in vitro
This study investigated the inhibitory effect of Lactobacillus sp. OPK‐3 isolated from kimchi (Korean traditional fermented food) ( L . sp. OPK‐3) on obesity and inflammatory cytokines in preadipocyte 3T3L‐1, RAW 264.7 macrophage cell and C57BL/6J mice. 3T3‐L1 cells were treated with L . sp. OPK‐3 significantly reduced lipid accumulation. The mRNA levels of adipocyte‐specific marker genes are regulated by L . sp. OPK‐3. In vivo , five groups of C57BL/6J mice fed with a ND (Normal diet control), HFD (High fat diet control), HFD+ornithine, HFD+ L. brevis , and HFD+ L. sp. OPK‐3 for 12weeks. All lactic acid bacteria supplemented groups had lower body weights and, more specifically, resulted in the Lactobacillus sp. OPK‐3 supplement group. Lipid profiles and inflammatory cytokines were improved by Lactobacillus sp. OPK‐3 compared with HFD control, ornithine and L. brevis groups. These results confirmed mRNA expression of enzymes related to lipid metabolism and inflammatory cytokines can be affected by the lactic acid bacteria in liver and adipose tissue. These results indicate that lactic acid bacteria from kimchi improve obesity and inflammatory cytokines through a modulator of lipid metabolism. This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government(MEST)” (NRF‐2008‐331‐E00371)