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Hypopigmentation of Shintoe black rice ( Oryza sativa L.) and its compound, momilactone B, in B16 murine melanocyte
Author(s) -
Lee Ji Hae,
Jun Heejin,
Cho BoRam,
Hoang Minh Hien,
Lee SungJoon
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.435.8
Grains contain various of phytochemicals that could modulate cellular melanogenesis. We investigated the inhibitory effects of a newly bred rice variety called Sinto black rice (SBR, Oryza sativa L.) and its component, momilactone B (MB) on melanogenesis in B16 murine melanocyte. MB inhibited mushroom tyrosinase activity (−23%), a key enzyme in melanogenesis, and reduced cellular melanin contents (−18%) in B16 cell. Quantitative real‐time PCR and immunoblot analyses quantified key genes and proteins expression in melanogenesis after either SBR extract or MB treatment. The protein expressions of TRP1 (−76%), TRP2 (−70%), and MITF in MB treated group and activated ERK phosphorylation (63%) compared with controls. The gene expression results showed similar trends. SBR extracts showed similar results as well. In conclusion, MB may be an active hypopigmentation compound in SBR extract. The reduced synthesis of cellular melanin may be mediated by dual mechanism: direct inhibition of tyrosinase enzyme and the activation of of ERK phosphorylation.