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SIMULTANEUS DETECTION OF OXIDIZED AND NITRATED OLEIC ACID METABOLITES IN PATIENTS WITH CHRONIC LIVER DISEASE
Author(s) -
Batkai Sandor,
Zoerner Alexander A.,
Pischke Sven,
Wedemeyer Heiner,
Jordan Jens,
Tsikas Dimitrios
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.366.1
Endogenous oxidative and nitrative metabolites of oleic acid ( cis ‐9,10‐epoxy‐oleic acid ( cis ‐EpOA), 9‐ and 10‐nitro‐oleic acid (9‐ NO 2 OA and 10‐NO 2 OA)) may be formed in the liver via enzymatic and non‐enzymatic routes. Since oxidative‐nitrative stress has been implicated in chronic liver disease, analysis of oxidative‐nitrative metabolites of oleic acid may provide more detailed pathomechanistic insight. Here we report a simple and reliable GC‐MS/MS method for the simultaneous quantitative determination of oxidized and nitrated metabolites in clinical samples. In a cohort of patients with end‐stage liver disease on the waiting list for liver transplantation, the plasma levels of cis ‐EpOA were significantly lower than in the controls, even as 9‐ and 10‐NO 2 OA levels were similar to the control group. cis ‐EpOA levels well correlated with liver function. All three oleic acid metabolites were also measurable in liver tissue samples from patients. In summary, we have developed and validated a method for simultaneous measurement of oxidative‐nitrative oleic acid metabolites in plasma and tissue. We confirmed that plasma levels of cis ‐EpOA levels are decreased in patients with severe hepatic dysfunction. Further studies are warranted to evaluate these oxidative‐nitrative fatty acid metabolites in chronic liver disease patients as potential markers of disease progression and severity.