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The Acute Effect of Ingesting a Quercetin‐Based Supplement on Exercise‐Induced Changes in Innate Immune Function
Author(s) -
Nieman David Christopher,
Konrad Manuela,
Henson Dru,
Kennerly Krista,
Jin Fuxia,
Deng Jun,
Jiang Qi,
Shanely R. Andrew
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.352.4
This study tested the acute influence of a quercetin‐based supplement on innate immune function in runners following an intense 2‐h run. In this randomized, crossover study, 20 runners (N=11 males, N=9 females, age 38.4±2.1 y) completed two 2‐h treadmill runs at 70% VO 2max (3 weeks apart). Subjects ingested either four Q‐chews or placebo chews (PL) 15 min prior to the runs. The four Q‐chews provided 1000 mg quercetin, 120 mg EGCG, 400 mg isoquercetin, 400 mg EPA and DHA, 1000 mg vitamin C, and 40 mg niacinamide. Subjects provided blood samples 30 min before, immediately following and 1‐h post‐exercise, and were analyzed for granulocyte and monocyte phagocytosis (GR‐PHAG and MO‐PHAG) and oxidative burst activity (GR‐OBA and MO‐OBA) using a flow cytometric procedure with FITC‐labeled Staphylococcus aureus bacteria and hydroethidine (Molecular Probes, Eugene, OR). All blood samples were prepared at the same ratio of 8 bacteria/phagocyte. Plasma quercetin was elevated from 80.0±26.0 to 6,337±414 (post‐exercise) and 4,324±310 μg/L (1‐h post‐exercise) after ingestion of Q‐chews compared to no change in PL. Exercise caused significant increases in both GR‐PHAG (30%) and MO‐PHAG (26%), and decreases in GR‐OBA (39%) and MO‐OBA (18%), but no differences in the patterns of change were measured between Q‐chew and PL trials. In conclusion, acute ingestion of Q‐chews 15 min before heavy exertion caused a strong increase in plasma quercetin levels but did not counter post‐2‐h run changes in innate immune function.