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Dietary Epigallocatechin‐3‐gallate (EGCG) supplementation reduces clinical symptoms and pathology associated with experimental autoimmune encephalomyelitis (EAE) in mice via altered balance among CD4+ T cell subsets
Author(s) -
Wang Junpeng,
Ren Zhihong,
Xu Yanmei,
Meydani Simin Nikbin,
Wu Dayong
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.352.1
Subject(s) - experimental autoimmune encephalomyelitis , autoimmunity , immunology , rar related orphan receptor gamma , multiple sclerosis , immune system , medicine , t cell , experimental pathology , autoimmune disease , spleen , biology , foxp3 , in vivo , antibody , microbiology and biotechnology
Studies suggest that green tea ingredient EGCG may be beneficial in reducing pathogenesis of autoimmunity; however, the underlying mechanisms are not well understood. We utilized EAE, an animal model for human multiple sclerosis, to determine effect of EGCG on autoimmunity and its underlying mechanisms. Female C57BL/6 mice were fed EGCG (0, 0.15, 0.3, and 0.6% in the diet) for 30 d and then immunized with specific antigen MOG 35–55 . All differences claimed below are statistically significant at p<0.05 or smaller. Results showed that EGCG dose dependently mitigated the clinical symptoms and pathology (leukocyte infiltration and demyelination in CNS), and inhibited antigen‐specific T cell proliferation and delayed type hypersensitivity skin response. In addition, EGCG reduced production of IFN‐γ, IL‐17, IL‐6, TNF‐α, & IL‐2, decreased Th1 and Th17, and increased Treg populations in lymph nodes, spleen, and CNS. Furthermore, EGCG inhibited expression of T‐bet & RORγT, the specific transcription factor for Th1 and Th17 differentiation, respectively. These results indicate that EGCG may suppress autoimmune response by inhibiting immune cell infiltration and modulating the balance among pro‐and anti‐autoimmune T cell subsets. These findings substantiate the beneficial effect of EGCG in autoimmune disease and identify a novel mechanism for its effect. Supported by Agriculture and Food Research Initiative Grant #2010‐65200‐20360 from the USDA National Institute for Food and Agriculture and USDA contract #58‐1950‐7‐707.