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Effects of Duration of Dose and Treatment of Dietary Eicosapentaenoic Acid (EPA) and Stearidonic Acid (SDA) on EPA Levels in Red Blood Cell (RBC) Membranes
Author(s) -
Mukherjea Ratna,
Krul Elaine S,
Liu Peter,
Lemke Shawna L,
Taylor Mary L,
Goldstein Daniel A,
Su Hong,
Harris William S,
Maki Kevin C
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.349.6
SDA is a product of delta‐6 desaturase, the rate‐limiting enzyme in the conversion of alpha‐linolenic acid (ALA) to EPA. Soybean oil enriched with SDA, produced through biotechnology, is a food source of omega‐3 fatty acids. Previous studies have demonstrated an increase in %EPA in RBC with SDA enriched soybean oil and SDA ethyl ester. The objective of the present study was to assess the effects of different doses of and durations of treatment with encapsulated ethyl esters of SDA and EPA on levels of EPA in RBC. Healthy subjects were randomized to SDA (0.5, 1.5, 3.0 or 6.0 g/d), EPA (0.5, 1.5, 3.0 g/d) or control (encapsulated safflower oil 0.5g/d) for 12 wks; 121 subjects completed the study. No treatment‐specific adverse events were observed. Repeated Measures Analysis showed that SDA and EPA increased (p<0.001) %EPA in RBC and omega‐3 index (O3I; RBC EPA + DHA) compared to control. O3I was higher than control at 12 weeks (p<0.05) with SDA doses of 1.5–6g/d. The relative efficiency of conversion varied across dose with a value of 1 to 3.87 (EPA:SDA) at an SDA dose of 1.5 g/d. The relationship between time and EPA enrichment in RBCs with both SDA and EPA appears to follow a first order kinetic model. In conclusion, SDA increased RBC %EPA and the O3I in a dose‐and‐time dependent manner. Study was supported by Solae LLC and Monsanto Company.