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Neutrophil‐induced epithelial repair in bronchiolar explants
Author(s) -
Hyde Dallas M,
Putney Lei,
Usachenko Jody,
Schelegle Ed,
Van Winkle Laura,
Avdalovic Mark
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.300.2
Subject(s) - explant culture , immunohistochemistry , pathology , in vivo , airway , epithelium , respiratory tract , respiratory epithelium , andrology , in vitro , biology , immunology , medicine , respiratory system , anatomy , surgery , biochemistry , microbiology and biotechnology
The purpose of this study was to investigate the efficacy of airway explants in the role of neutrophil‐enhancement of epithelial repair in response to ozone‐induced epithelial injury. Wistar rats were exposed to ozone for 8 hours to create epithelial necrosis of the terminal bronchioles. Epithelial repair of the terminal bronchioles was compared between rats and explant bronchioles from rats over 24 hours postexposure. Airways were microdissected and placed in tissue culture media with BrdU in an air liquid interface environment and evaluated at 8, 12, 16, 20 and 24 hours later using immunohistochemistry and morphometry. Terminal bronchiolar explants from rats showed a very similar pattern of BrdU labeling postexposure to in vivo labeling in rats, but with about a 4 hour delay. Bronchiolar explants from ozone‐exposed rats with neutrophils primed for migration provide multiple target airways per rat for experimental intervention to investigate mechanisms of epithelial repair in a controlled environment. Supported by Grant NIEHS ES00628.