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Retinoic acid induces apoptosis in activated canine neutrophils
Author(s) -
Channabasappa Shankaramurthy,
Stewart Sarah,
Caldwell Sarah,
Carr Anthony,
Singh Baljit
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.300.1
Subject(s) - retinoic acid , apoptosis , chemistry , microbiology and biotechnology , cancer research , biology , biochemistry , gene
Neutrophils are the first cells to be recruited to the site of injury or infection. Activated neutrophils have prolonged lifespan and release cytotoxic contents resulting in considerable tissue injury and delayed repair of inflamed tissue. Neutrophil‐induced tissue injury is central to the pathogenesis of many inflammatory diseases. Induction of controlled apoptosis of activated neutrophils at the inflammatory site can hasten the resolution of inflammation. In this experiment we examined the effects of retinoic acid on apoptosis of normal and activated canine neutrophils using light microscopy, electron microscopy, terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay, caspase‐3 assay and Annexin V staining. Our results indicated that treatment with 1 μg/ml bacterial lipopolysaccharide (LPS) for 12 and 36 hours significantly reduced the percentage of apoptotic cells compared to untreated cells. The effect of LPS on neutrophil apoptosis was abolished in neutrophls simultaneously treated with retinoic acid (1 and 5 μM). Pro‐apoptotic effects of retinoic acid on activated neutrophils suggest new mechanism of anti‐inflammatory effect of retinoic acid. Grant Funding Source : WCVM Vitamin Class Action Settlement Fund