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Comparison of the response to broccoli sprouts or broccoli supplement consumption in human subjects
Author(s) -
Clarke John,
Riedl Ken,
Bella Deborah,
Schwartz Steven,
Stevens Jan,
Ho Emily
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.234.7
Subject(s) - consumption (sociology) , food science , horticulture , chemistry , biology , art , aesthetics
Broccoli consumption may reduce the risk of various cancers and many broccoli supplements are available. We sought to assess possible differences in metabolism and bioactivity of isothiocyanates (ITC) between a whole food and a broccoli supplement. First, subjects were randomized into a 7‐d feeding study where subjects consumed either fresh broccoli sprouts or supplement with equivalent glucoraphanin content each morning. Metabolites of two major ITCs, sulforaphane (SFN) and erucin (ERN) were assessed in urine samples during the 7‐d regimen and up to 48 h following 7‐d consumption by LC‐MS. Second, to directly compare effects between the whole food and supplement in the same individual, subjects were randomized into a single dose cross‐over study with broccoli sprouts and broccoli supplements with 4wk washout period between. The total amount of SFN and ERN metabolites was greater and the peak concentration occurred sooner in subjects who consumed fresh broccoli sprouts compared to subjects who consumed the supplement in both studies. Possible in vivo interconversion of SFN to ERN was also observed. Histone deacetylase activity in peripheral blood mononuclear cells was inhibited only in subjects who consumed sprouts. In conclusion, consumption of broccoli supplements resulted in differential effects on ITC concentrations and kinetics and molecular targets compared to the whole food. Funding: NIH CA122906 Grant Funding Source: NIH CA122906