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Human Urinary Metabolic Profile of Tea Polyphenols: Potential Exposure Markers
Author(s) -
Sang Shengmin
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.234.1
Subject(s) - polyphenol , chemistry , urine , metabolomics , electrospray ionization , chromatography , tandem mass spectrometry , sulfation , metabolome , catechin , liquid chromatography–mass spectrometry , biochemistry , mass spectrometry , food science , antioxidant
Tea is rich in polyphenols and has a variety of biological activities. In order to better understand the biological effects of tea constituents on human health, markers for their exposure and their metabolic fates are needed. We have studied the human urinary metabolic profile of tea polyphenols using liquid chromatography/electrospray ionization tandem mass spectrometry with data‐dependent acquisition. With data‐dependent MS/MS analysis by collecting the MS 2 and MS 3 spectra of the most intense ions in the sample, we identified more than twenty metabolites of tea polyphenols from human urine samples. Our results indicate that phenolic groups of methylated tea catechins (or glucuronidated or sulfated tea catechins) can be further methylated, glucuronided and/or sulfated to form multiple conjugated metabolites. Our recent approach to establish the potential exposure markers of tea polyphenols using targeted metabolomics will also be discussed.