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Edible mushrooms promote gut immunity by enhancing IL‐23 and IL‐6 secretion in dextran sodium sulfate (DSS)‐treated mice
Author(s) -
Christopher Lawrance,
French Christine,
Traore Djibril,
Kuvibidila Solo
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.222.8
Subject(s) - immune system , secretion , immunity , microbiology and biotechnology , spleen , biology , cytokine , interleukin 10 , immunology , andrology , chemistry , medicine , endocrinology
IL‐23, a novel cytokine secreted by dendritic cells, is involved in host defense against gut pathogens such as Vibrio cholerae and promotes gut immunity through IL‐23 and IL‐17 axis. Together with IL‐6, IL‐23 promotes CD4 + T cell differentiation to Th17 cells that secretes IL‐17. Although mushrooms possess anti‐pathogen properties, their effect on IL‐23 is unknown. We investigated whether one of the mechanisms by which mushrooms protect from infection is through IL‐23 induction. C57BL/6 adult female mice were fed either the AIN76 diet or the same diet fortified with 5% white button mushrooms (WBM), portabella, or shiitake (n=16/group). For cytokine induction, 8 mice/group received 3% DSS in drinking water during the last 5 days of the 6‐week feeding period. In DSS‐untreated mice, mushrooms increased IL‐23 plasma levels (2–15 fold); but decreased those of IL‐6 (45%–98%; p<0.05). In DSS‐treated mice, mushrooms increased IL‐6 1.4–3.7 fold and IL‐23 10–50 fold (p <0.05). DSS alone increased plasma levels of IL‐6 and IL‐23 by ≥ 4 fold (p<0.05), with the highest levels observed with WBM. However, mushroom‐fortification did not increase in vitro IL‐6 secretion by Con A or LPS (2.5 μg/ml) activated spleen cells suggesting a main role of mushrooms on gut immune cells. To the best our knowledge, this is the first report documenting the health benefits of mushrooms through IL‐23. The mechanism of IL‐23 reduction is under investigation. Supported by Mushroom Council/USDA, OSU startup funds, and OCAST . Grant Funding Source : OCAST, Mushroom Counil, USDA, Oklahoma State University