Premium
Protective Effects of Veskamide, Enferamide, Becatamide, and Oretamide on H2O2‐Induced Apoptosis of PC‐12 Cells.
Author(s) -
Park Jae B
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.213.7
Subject(s) - apoptosis , depolarization , membrane potential , chemistry , mitochondrion , prophase , microbiology and biotechnology , neuroprotection , inner mitochondrial membrane , chromatin , programmed cell death , cell , membrane , caspase 3 , biophysics , biochemistry , biology , pharmacology , dna , meiosis , gene
Neurodegenerative disease is a devastating disease progressively deteriorating the structure and/or function of neurons, eventually leading to cell death. In this study, veskamide, enferamide, becatamide, and oretamide found in plants were prepared by chemical syntheses and their potential neuroprotective effects on H2O2‐induced apoptosis in PC‐12 cells were investigated. The syntheses were relatively simple with the yields of more than 43%. Using NMR spectroscopic methods, the chemical structures of veskamide, enferamide, becatamide, and oretamide were confirmed. The decreasing order of the protective effects on H2O2‐induced apoptosis was becatamide > enferamide > oretamide > veskamide. In fact, becatamide suppressed H2O2‐induced mitochondrial membrane depolarization in a dose‐dependent manner. At 10 μM, becatamide maintained mitochondrial membrane depolarization at 16% compared to 51% in H2O2‐treated PC‐12 cells (P<0.05). Also, at the same concentration, becatamide inhibited H2O2‐induced caspase‐9 activation and caspase‐independent chromatin condensation by 68 % (P<0.05) and 73% (P<0.05), respectively. These data suggest that becatamide may provide protective effects on H2O2‐induced apoptosis of PC‐12 cells via protecting mitochondrial membrane integrity, thereby suppressing caspase‐9 activation and chromatin condensation. This study was funded by USDA.