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Cell and Extracellular Matrix Dynamics in Skeletal Tissues
Author(s) -
Dallas Sarah Louise
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.185.1
Subject(s) - osteocyte , extracellular matrix , fibronectin , microbiology and biotechnology , osteoblast , bone cell , live cell imaging , matrix (chemical analysis) , chemistry , bone remodeling , bone matrix , biophysics , cell , biology , anatomy , biochemistry , in vitro , cartilage , genetics , chromatography
Much of our understanding of mineralized tissue biology has come from the use of static imaging approaches, such as light and electron microscopy, combined with chemical and biochemical analysis and/or molecular genetic approaches. However, the biological processes occurring during bone formation, remodeling and repair are dynamic events that have the added dimension of time. The goal of our research is to understand the dynamic interactions of cells in bone and in particular the dynamic processes occurring during assembly of bone extracellular matrix and the transition from the osteoblast to the embedded osteocyte. To address this, we have used live cell imaging techniques in conjunction with fluorescent probes for bone ECM molecules, such as collagen and fibronectin, and fluorescent reporters for the osteoblast and osteocyte lineages. These studies have revealed the highly dynamic properties of bone cells and their cell‐cell interactions in bone and have revealed the dynamic nature of bone matrix assembly and its integration with cell motile function.