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Dynamic analysis of organogenesis: Let's start with the heart
Author(s) -
Lansford Rusty
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.181.4
Subject(s) - quail , vasculogenesis , biology , organogenesis , angiogenesis , microbiology and biotechnology , transgene , embryo , embryonic heart , anatomy , embryonic stem cell , genetics , endocrinology , stem cell , gene , progenitor cell
My group investigates the fundamental principles that guide how cells self organize through collective interactions to bring about changes in embryonic form and function. To this end, we developed transgenic, fluorescent protein (FP) expressing Coturnix quail as an experimental system in order to dynamic image amniote embryogenesis. To increase our understanding of how vascular cells and their precursors are precisely patterned in space and time, we are using dynamic computational imaging, in vivo, in conjunction with a new array of elegant molecular tools tailored for use in wild‐type and transgenic quail embryos. In particular, we are using the Tg(tie1:H2B‐eYFP) and Tg(pgk:H2B‐chFP) quail to study vasculogenesis, angiogenesis, and cardiogenesis. We are studying the interplay between genetics and hemodynamics that effect formation of the four‐chambered heart. We strive to establish temporal and spatial relationships between genetic, biophysical and morphological determinants required for heart formation.