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Analysis of endothelial cell movements during aortae development
Author(s) -
Rupp Paul A.,
Lansford Rusty,
Czirok Andras
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.177.1
Subject(s) - quail , vasculogenesis , anatomy , biology , in vivo , microbiology and biotechnology , motility , in vitro , medicine , endothelial stem cell , endocrinology , biochemistry
The migratory behavior of endothelial cells (ECs) during vasculogenesis was investigated in vivo using a TIE1:H2B‐YFP transgenic quail line. Similarly to studies in which ECs were labeled with QH1 antibody, cells migrated in a lateral to medial direction and then moved anteriorly upon arrival at the dorsal aortae. Coordinated, but unoriented EC motility at the aortae becomes an intensive, organized migration against flow (towards the heart) after the onset of circulation. By perturbing blood flow, we show that this migratory behavior is likely guided by hemodynamic forces ‐‐ as suggested by previous in vitro studies. Funded by NIH RO1 HL087136.