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Ethanol effects on cell cycle related genes in regenerating rat liver
Author(s) -
Juskeviciute Egle,
Vadigepalli Rajanikanth,
Sonnenahalli Harikrishnadhar,
Hoek Jan B.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.115.3
Subject(s) - liver regeneration , ethanol , calorie , cell cycle , gene expression , biology , gene , medicine , analysis of variance , andrology , transcriptome , liver cell , endocrinology , chemistry , biochemistry , regeneration (biology) , microbiology and biotechnology
In this study, we analyzed the effects of chronic alcohol consumption on liver repair and regeneration after partial hepatectomy (PHx) with a focus on cell cycle related genes. Rats were fed a liquid diet containing 36% of total calories derived from ethanol for 5 weeks; corresponding pair‐fed calorie‐matched controls were fed diets in which ethanol calories were replaced either by carbohydrate or by fat. After 5 weeks, rats were subjected to 70% PHx and liver samples were collected at 1, 6 and 24h after the surgery. The excised liver samples at t=0 served as within‐animal controls. We used Affymetrix Rat Gene 1.0 ST arrays with ~25000 probe sets to obtain global gene expression data from each liver sample (n=4 replicate rats, 72 arrays total). Analysis of the gene expression data using a three‐way ANOVA revealed approximately 6500 genes as affected by PHx in at least one of the diet groups at either 1, 6 or 24h post PHx (false discovery rate < 15%). Functional annotation analysis using indicated that chronic ethanol consumption blunted cell cycle related gene responses at 24h post PHx. This correlated with a suppression of BrdU incorporation in liver cells in the regenerating liver from ethanol‐fed animals. The effects of ethanol treatment on the expression levels of cell cycle related genes in the regenerating liver were validated using BioMark Dynamic Array. Supported by AA008714 , AA014986 , AA017261 , AA016919 , and AA018873.