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Oxidative Stress is Strain Dependent in Bleomycin‐induced Pulmonary Fibrosis
Author(s) -
Porto Luis Cristovao,
Silva Marco Aurelio S,
Trajano Eduardo,
Pires Karla Maria P,
Lanzetti Manuella,
Martins Vanessa,
Caetano Maurício,
Machado Mariascimento,
Sternberg Cinthya,
Benjamim Claudia,
Zin Walter A,
Valenca Samuel S
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.114.8
Subject(s) - oxidative stress , bleomycin , catalase , strain (injury) , chemistry , pulmonary fibrosis , pathogenesis , medicine , endocrinology , saline , lung , c57bl/6 , oxidative phosphorylation , immunology , pathology , microbiology and biotechnology , biology , biochemistry , chemotherapy
Oxidants play an important role in pulmonary fibrosis. We investigated the oxidative stress (OS) markers in the lungs of different mouse strains. Male C57BL/6, DBA/2 and BALB/c mice were divided in Control group (50μl saline; i.t., N = 10 each strain) and Bleomycin group [B] (0.1 U/kg i.t., N = 10 each strain). After 21 days, lung mechanics was assessed and then mice were killed; lungs were homogenized for OS markers measurement. Statistical analysis compared B groups with their respective controls. Elastance, and both peripheral and central lung resistance were only increased in C57BL/6 group (p<0.05). MDA levels were increased in the C57BL/6‐B group (p<0.01), but were decreased in DBA/2 and BALB/c‐B groups (p<0.0001). Both activity and expression of catalase were increased in C57BL/6‐B groups (p<0.01), but were decreased in BALB/c‐B groups (p<0.01). SOD activity increased in C57BL/6 and BALB/c‐B groups (p<0.01). Nitrite increased in C57BL/6 and BALB/c (p<0.01) groups. Our results show C57BL/6 mice are more susceptible to redox imbalance and oxidative damage. Also, BALB/c strain show some degree of redox imbalance; however DBA and BALB/c strains seem to be resistant to oxidative damage.Financial Support: CAPES, UERJ, CNPq