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A Comparison Of Two Surgical Methods For Chronic Measurement Of Blood Pressure In Juvenile Rats Through Adulthood
Author(s) -
Bogie Heather Marie,
Sarazan R. Dustan
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1126.5
Subject(s) - juvenile , catheter , medicine , femoral artery , animal model , transmitter , blood pressure , artery , surgery , anesthesia , biology , channel (broadcasting) , electrical engineering , engineering , genetics
Collecting chronic blood pressure (BP) from juvenile animals through adulthood can provide a number of research benefits. The number of animals used, coinciding surgical support, and animal variability can all be reduced by using one BP transmitter model throughout the animal's lifetime. The PA‐C40 transmitter (DSI), typically used in rats and similar species is too large in size and catheter diameter for smaller rodent arteries. However, the PA‐C10 transmitter (DSI), which is typically implanted in mice or similar‐sized species, was chosen as a possible alternative to the larger PA‐C40. The PA‐C10 was implanted in 12 male, juvenile rats (SD, ~90 grams) to determine the most suitable placement for the catheter and transmitter body to retain catheter patency during the growth period. Two separate surgical procedures were evaluated including direct aortic cannulation with intraperitoneal (IP) transmitter placement in six rats and direct femoral artery cannulation with subcutaneous (SQ) transmitter placement in six rats. BP was monitored and collected three days per week for a total of 16 weeks. Catheter patency was determined by analyzing the collected data and visualizing in vivo catheter placement. At the end of the study, 11 of the 12 rats had viable BP signals. Therefore, BP measurement in growing rats, from juvenile to adult, is a viable experimental design for cardiovascular researchers.