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β‐Hydroxy‐β‐Methylbutyrate (HMB) Improves Body Composition and Myofiber Dimensions in Mice during Normal Physical Conditioning Not during Catabolic Conditions
Author(s) -
Kim JeongSu,
Henning Paul C,
Park BongSup,
Lee SangRok,
Bakhshalian Neema,
Masad Ihssan S,
Wilson Jacob M,
Park YoungMin,
Arjmandi Bahram H,
Grant Samuel C
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.1126.1
Subject(s) - lean body mass , catabolism , medicine , endocrinology , myocyte , chemistry , composition (language) , metabolism , body weight , linguistics , philosophy
Lean mass (LM) decreases in soldiers due to the arduous training performed in garrison and during combat operations. We investigated the impact of HMB on myofiber dimensions and body composition during normal training vs. catabolic conditions in male C57BL/6 mice. Sixty one mice underwent a 4‐wk run‐in‐phase to simulate basic combat training and were then divided into 4 groups (n=10/group): AL= ad lib fed+exercise (1h/d for 3d/wk), ALH=AL+HMB (0.5 g/kg BW/day), C=caloric restricted (−30% of ad lib groups)+exercise (2 km/d, 6d/wk) and CH=C+HMB to simulate 6 wks of normal and catabolic conditions in soldiers. We utilized dual x‐ray absorptiometry [LM and fat mass (FM)] and magnetic resonance (myofiber dimensions) pre‐ and post‐experiment. ALH exhibited a greater LM compared to all other groups. LM in ALH was greater (+17%) than AL at post. LM in C was greater (+21%) than CH at post. FM increased in AL (+25% vs pre). FM in the ALH was lower (−11%) than AL at post. FM decreased in both the C (−56%) and CH (−38%) from pre. There was an increase in fractional anisotropy (+44% vs pre) and apparent diffusion coefficient (ADC, +11% vs pre) in AL. ADC also increased (+11%) in C. While ALH showed no change in a myofiber cross sectional area (λ3), AL decreased in λ3 (−30%) from pre. Our in vivo analyses reveal that HMB improves body composition and myofiber dimensions in mice during normal physical conditioning but not catabolic conditions.